American Qualex

Primary Antibodies

These products were developed for assay development and research using the highest standards of quality and excellence. American Qualex offers a complete line of quality antisera that is suitable for a variety of applications. Primary antibodies are sold for bulk or research use. Different grades available include RIA, Immunohistochemical and Nephelometric.
  • RIA grade, which is provided by volume or test aliquots, is generally high titer, high specificity material suitable for radioimmunoassay.
  • Immunohistochemical grade, ideal for indirect IFA or EIA staining techniques, is provided by the milliliter (ml) or by the milligram (mg).
  • Nephelometric grade, for precipitating and other techniques is also sold by volume.

  • Samples are generally available for a fee determined by the antigen and titer. Please inquire for pricing and current availability of products.

    American Qualex

    Secondary Antibodies

    Adsorbed antibodies are typically adsorbed against immunoglobulins from up to 14 different species.

    AMERICAN QUALEX Affinity Purified Secondary Antibodies are tested for purity and specificity by immunoelectrophoresis and immunoprecipitation. Conjugates are tested for immuno-enzyme activity by direct enzyme immunoassay. Even product includes a product data sheet which contains all the pertinent information as well as suggested working dilutions.

    Unlabeled Affinity-purified antibodies are supplied at a concentration of 2.0 mg/mi in 0.01 M phosphate buffered saline (PBS), pH 7.6 with 0.1% sodium azide as a preservative. Store at -20 C

    Alkaline Phosphatase (Calf intestine) labeled antibodies are supplied at 0.5 mg/ml in 0.05 M Tris-HCl, ph 8.0 with 0.001 M MgC12, 1% BSA and 0.1% sodium azide. Suggested working dilution is approximately 1:5000 for direct ELISA. Store at 4C.

    Horseradish Peroxidase (HRPO) labeled antibodies are provided at 1.0 mg/ml in 0.01 M PBS, pH 7.6 containing 10% mannose, 2% sucrose, and 1% BSA as stabilizing reagents and 0.02% thimerosal as a preservative. Suggested working dilution is approximately 1 :10,000 for direct ELISA. Store at 4C

    Beta-Galactosidase E. coli) labeled antibodies are packaged at 0.25 mg/ml in 0.05 M PBS, pH 7.0 with 1% BSA and 0.1% sodium azide. Suggested working dilution for fluorescent EIA is approximately 1:3,000, for ELISA 1:1,000. Store at 4C.

    AMERICAN QUALEX ENZYME LABELED AFFINITY PURIFIED ANTIBODIES PROMISE: Enzyme Immune Adsorbant Assays (ELISA), Staining, and Blotting applications require conjugates, that are high in sensitivity, specificity, and working dilution. AQ offers antibodies labeled with Horseradish Peroxidase, Alkaline Phosphatase, and Beta-Galactosidase. Each enzyme is conjugated to antibodies using the method that will best optimize its performance. AQ features a bidirectional method of conjugating Alkaline Phosphatase and Peroxidase. This procedure yields a highly sensitive conjugate that helps in retaining optimal antibody and enzyme activity for assays done on solid support, such as polystyrene or nitrocellulose . Whole molecules and F(ab')2 fragments are available cross-adsorbed for species specificity, ideal for species sensitive assays.